Factor VHI : C Concentrate Purified From Plasma Using Monoclonal Antibodies : Human Studies

Conventional clotting factor concentrates have, until recently. been “of intermediate purity.” containing <1 % of the coagulation factor, and >99% extraneous plasma proteins such as fibrinogen, fibronectin. gamma globulins. and traces of many others. We report here the results of a new factor VIII concentrate that is purified from human plasma using a mouse monoclonal antibody to factor Vlll:vWF in an affinity chromatography system. The resultant concentrate has an activity of between 3.000 and 5.000 U/mg protein before albumin is added as a stabilizer. Seven patients with severe hemophilia A and no inhibitor who were positive for antibody to human immunodeficiency virus (HIV) have been treated solely with this concentrate for over 24 months. Factor usage in these patients has ranged from 6i1 U/kg/yr to 2,022 U/kg/yr. These